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Construction and expression of hepatitis B virus vector encoding TC-tagged core protein

Yuanyuan LIN MD, Xiaoming CHENG MS, Yuhu SONG MD, Peiyuan LI MD, Ying CHANG MM, Jinjian YAO MD, Jusheng LIN MD, PhD, Li ZHOU PhD, Leiming XU PhD,

《医学前沿（英文）》 2009年 第3卷 第4期   页码 396-402 doi: 10.1007/s11684-009-0056-z

摘要： Virus tagged with green fluorescent protein (GFP) contributes to the visualization and study of the virus in living cells. However, the hepatitis B virus (HBV) particle, which is a compact virion with limited internal space, cannot be incorporated with GFP tag as a large fragment. It was recently reported that protein genetically inserted with a smaller size tetracysteine (TC) tag could be specially labeled by a biarsenical fluorescent dye in living cells. In this study, we constructed a recombinant HBV vector encoding TC-tagged core protein for biarsenical labeling of HBV virion. TC tag was genetically inserted near the immunodominant c/e1 site of HBV core protein by mutagenesis. Western blot and enzyme-linked immunosorbent assay (ELISA) analysis showed that the TC-tagged core protein, hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) could be expressed in cells transfected with the recombinant HBV vector, which is similar to the cells transfected with wild-type HBV vector. Reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis showed that HBV virion formation was affected by the genetic insertion of TC tag into core protein in some degree, but cells transfected with the HBV vector could still produce HBV virions incorporated with TC-tagged core proteins. Taken together, the recombinant HBV vector can serve as a useful tool to produce HBV virions incorporated with TC-tagged core proteins to be fluorescently labeled by biarsencial dye for visualizing and studying HBV in living cells.

关键词： hepatitis B virus     vector     tetracysteine tag     core protein    

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脂肪变性诱发肝癌发生机制——HCV核心基因转基因小鼠的经验教训 Review

Pan Diao, Fangping Jia, Xiaojing Wang, Xiao Hu, Takefumi Kimura, Takero Nakajima, Toshifumi Aoyama, Kyoji Moriya, Kazuhiko Koike, Naoki Tanaka

《工程（英文）》 2021年 第7卷 第12期   页码 1797-1805 doi: 10.1016/j.eng.2021.08.019

摘要：

丙型肝炎病毒（HCV）感染是全球慢性肝炎、肝硬化和肝细胞癌（HCC）的主要病因。在HCV的结构蛋白中，HCV核心蛋白具有调控基因转录、脂质代谢、细胞增殖、细胞凋亡和自噬的能力，所有这些都与HCC的发展密切相关。携带HCV核心基因的转基因小鼠表现出与慢性丙型肝炎患者的临床特征相似的年龄依赖性胰岛素抵抗、肝脂肪变性和HCC。一些饮食习惯的调整，包括限制热量和富含饱和脂肪酸（SFA）、反式脂肪酸（TFA）或胆固醇的饮食，被证明会影响HCV核心基因转基因小鼠的肝脏脂肪生成和肿瘤形成。这些饮食的改变除了调节肝纤维化过程和微环境外，还调节了肝细胞的应激和增殖，从而证实了饮
食习惯与脂肪变性相关的肝癌发生之间的密切联系。本文综述了HCV基因组转基因小鼠模型的研究结果，重点介绍了HCV核心基因转基因小鼠的研究结果，并讨论了HCV核心蛋白诱导脂肪变性和肝癌发生的机制，以及饮食习惯对脂肪变性所致肝癌的影响。

关键词： 脂肪变性     肝细胞癌     反式脂肪酸     饱和脂肪酸     饮食限制     丙型肝炎病毒核心蛋白    

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知识发现领域中当今面临的五类重大问题

杨炳儒

《中国工程科学》 2009年 第11卷 第4期   页码 76-83

摘要：

系统地总结与提出知识发现(KD)领域中当今面临的五类重大问题，它们是KD中的两大核心问题、两大猜想问题、主流发展中富有挑战性的问题、应用研究中的相关领域重大问题以及KD技术标准的制定问题，并给出其部分成果或具体分析。这五类问题密切相关，对它们的研究必将KD推向新的发展阶段,并在该领域内外产生深刻的影响。

关键词： KD核心问题     KD猜想     KD挑战性问题     蛋白质二级结构预测     KD技术标准    

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Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

《农业科学与工程前沿（英文）》 2016年 第3卷 第2期   页码 102-112 doi: 10.15302/J-FASE-2016100

摘要： Plants are frequently affected by pathogen infections. To effectively defend against such infections, two major modes of innate immunity have evolved in plants; pathogen-associated molecular pattern-triggered immunity and effector-triggered immunity. Although the molecular components as well as the corresponding pathways involved in these two processes have been identified, many aspects of the molecular mechanisms of the plant immune system remain elusive. Recently, the rapid development of omics techniques (e.g., genomics, proteomics and transcriptomics) has provided a great opportunity to explore plant–pathogen interactions from a systems perspective and studies on protein–protein interactions (PPIs) between plants and pathogens have been carried out and characterized at the network level. In this review, we introduce experimental and computational identification methods of PPIs, popular PPI network analysis approaches, and existing bioinformatics resources/tools related to PPIs. Then, we focus on reviewing the progress in genome-wide PPI networks related to plant–pathogen interactions, including pathogen-centric PPI networks, plant-centric PPI networks and interspecies PPI networks between plants and pathogens. We anticipate genome-wide PPI network analysis will provide a clearer understanding of plant–pathogen interactions and will offer some new opportunities for crop protection and improvement.

关键词： plant–pathogen interactions     systems biology     omics     plant immunity     protein–protein interaction     network    

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Postprocessor development for ultrasonic cutting of honeycomb core curved surface with a straight blade

《机械工程前沿（英文）》 2023年 第18卷 第1期 doi: 10.1007/s11465-022-0729-8

摘要： When ultrasonically cutting honeycomb core curved parts, the tool face of the straight blade must be along the curved surface’s tangent direction at all times to ensure high-quality machining of the curved surface. However, given that the straight blade is a nonstandard tool, the existing computer-aided manufacturing technology cannot directly realize the above action requirement. To solve this problem, this paper proposed an algorithm for extracting a straight blade real-time tool face vector from a 5-axis milling automatically programmed tool location file, which can realize the tool location point and tool axis vector conversion from the flat end mill to the straight blade. At the same time, for the multi-solution problem of the rotation axis, the dependent axis rotation minimization algorithm was introduced, and the spindle rotation algorithm was proposed for the tool edge orientation problem when the straight blade is used to machine the curved part. Finally, on the basis of the MATLAB platform, the dependent axis rotation minimization algorithm and spindle rotation algorithm were integrated and compiled, and the straight blade ultrasonic cutting honeycomb core postprocessor was then developed. The model of the machine tool and the definition of the straight blade were conducted in the VERICUT simulation software, and the simulation machining of the equivalent entity of the honeycomb core can then be realized. The correctness of the numerical control program generated by the postprocessor was verified by machining and accuracy testing of the two designed features. Observation and analysis of the simulation and experiment indicate that the tool pose is the same under each working condition, and the workpieces obtained by machining also meet the corresponding accuracy requirements. Therefore, the postprocessor developed in this paper can be well adapted to the honeycomb core ultrasonic cutting machine tool and realize high-quality and high-efficient machining of honeycomb core composites.

关键词： honeycomb core     straight blade     ultrasonic cutting     tool pose     postprocessor    

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Establishment and verification of a shrinking core model for dilute acid hydrolysis of lignocellulose

Cunwen WANG, Xiaoling DUAN, Weiguo WANG, Zihao LI, Yuanhang QIN

《能源前沿（英文）》 2012年 第6卷 第4期   页码 413-419 doi: 10.1007/s11708-012-0212-z

摘要： The kinetics of lignocellulose hydrolysis under the conditions of high temperature and dilute acid (mass fraction 0.05%) was investigated in this paper. By studying the reducing sugar concentration versus reaction temperature (170°C–220°C) and reaction time (150–1800 s) during the hydrolysis process of five kinds of crop straw (rice, wheat, cotton, rape and corn), the shrinking core model was established, and the differential equation of the model and its analytical solution were obtained. With a numerical calculation method, the kinetic equation was estimated, and the degradation of reducing sugar obeyed first-order kinetics was obtained. The calculated results from the equations agreed well with the original experimental data. The calculation by the model showed that the reducing sugar concentration increases as the size of the particles decrease, and the uniform particles increase.

关键词： lignocellulose     dilute acid hydrolysis     shrinking core model    

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Recent advances in gold-metal oxide core-shell nanoparticles: Synthesis, characterization, and their

Michelle Lukosi,Huiyuan Zhu,Sheng Dai

《化学科学与工程前沿（英文）》 2016年 第10卷 第1期   页码 39-56 doi: 10.1007/s11705-015-1551-1

摘要： Heterogeneous catalysis with core-shell structures has been a large area of focus for many years. This paper reviews the most recent work and research in core-shell catalysts utilizing noble metals, specifically gold, as the core within a metal oxide shell. The advantage of the core-shell structure lies in its capacity to retain catalytic activity under thermal and mechanical stress, which is a pivotal consideration when synthesizing any catalyst. This framework is particularly useful for gold nanoparticles in protecting them from sintering so that they retain their size, structure, and most importantly their catalytic efficiency. The different methods of synthesizing such a structure have been compiled into three categories: seed-mediated growth, post selective oxidation treatment, and one-pot chemical synthesis. The selective oxidation of carbon monoxide and reduction of nitrogen containing compounds, such as nitrophenol and nitrostyrene, have been studied over the past few years to evaluate the functionality and stability of the core-shell catalysts. Different factors that could influence the catalyst’s performance are the size, structure, choice of metal oxide shell and noble metal core and thereby the interfacial synergy and lattice mismatch between the core and shell. In addition, the morphology of the shell also plays a critical role, including its porosity, density, and thickness. This review covers the synthesis and characterization of gold-metal oxide core-shell structures, as well as how they are utilized as catalysts for carbon monoxide (CO) oxidation and selective reduction of nitrogen-containing compounds.

关键词： core-shell     characterization     core-shell structure     Different     stability    

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The role of protein kinase C epsilon in neural signal transduction and neurogenic diseases

null

《医学前沿（英文）》 2011年 第5卷 第1期   页码 70-76 doi: 10.1007/s11684-011-0119-9

摘要：

Protein kinase C epsilon (PKC ?) is one of major isoforms in novel PKC family. Although it has been extensively characterized in the past decade, the role of PKC ? in neuron is still not well understood. Advances in molecular biology have now removed significant barriers to the direct investigation of PKC ? functions in vivo, and PKC ? has been increasingly implicated in the neural biological functions and associated neurogenic diseases. Recent studies have provided important insights into the influence of PKC ? on cortical processing at both the single cell level and network level. These studies provide compelling evidence that PKC ? could regulate distinct aspects of neural signal transduction and suggest that the coordinated actions of a number of molecular signals contribute to the specification and differentiation of PKC ? signal pathway in the developing brain.

关键词： protein kinase C ?     signal transduction     neurogenic disease    

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Property-performance relationship of core-shell structured black TiO photocatalyst for environmental

《环境科学与工程前沿（英文）》 2023年 第17卷 第9期 doi: 10.1007/s11783-023-1711-3

摘要：

● Properties and performance relationship of CSBT photocatalyst were investigated.

关键词： Black TiO2     Core-shell structure     Property-performance relationship     Agro-industrial effluent     Environmental remediation    

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Wheat gluten protein and its impacts on wheat processing quality

Wujun MA, Zitong YU, Maoyun SHE, Yun ZHAO, Shahidul ISLAM

《农业科学与工程前沿（英文）》 2019年 第6卷 第3期   页码 279-287 doi: 10.15302/J-FASE-2019267

摘要：

Before the advent of the wheat genomic era, a wide range of studies were conducted to understand the chemistry and functions of the wheat storage proteins, which are the major determinants of wheat flour the suitability of wheat flour for various end products, such as bread, noodles and cakes. Wheat grain protein is divided into gluten and non-gluten fractions and the wheat processing quality mainly depends on the gluten fractions. Gluten provides the unique extensibility and elasticity of dough that are essential for various wheat end products. Disulfide bonds are formed between cysteine residues, which is the chemical bases for the physical properties of dough. Based on the SDS-extractability, grain protein is divided into SDS-unextractable polymeric protein (UPP) and SDS-extractable polymeric protein. The percentage of UPP is positively related to the formation of disulfide bonds in the dough matrix. In the wheat genomic era, new glutenins with long repetitive central domains that contain a high number of consensus hexapeptide and nonapeptide motifs as well as high content of cysteine and glutamine residues should be targeted.

关键词： wheat gluten     consensus motifs     disulfide bonds     SDS-unextractable polymeric protein     glutenins     gliadins     processing quality     storage protein    

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Experiment and calculation on seismic behavior of RC composite core walls with concealed steel truss

Wanlin CAO , Weihua CHANG , Changjun ZHAO , Jianwei ZHANG ,

《结构与土木工程前沿（英文）》 2009年 第3卷 第3期   页码 255-261 doi: 10.1007/s11709-009-0043-0

摘要： To improve the seismic performance of reinforced concrete core walls, reinforced concrete composite core walls with concealed steel truss were proposed and systemically investigated. Two 1/6 scale core wall specimens, including a normal reinforced concrete core wall and a reinforced concrete composite core wall with concealed steel truss, were designed. The experimental study on seismic performance under cyclic loading was carried out. The load-carrying capacity, stiffness, ductility, hysteretic behavior and energy dissipation of the core walls were discussed. The test results showed that the seismic performance of core walls is improved greatly by the concealed steel truss. The calculated results were found to agree well with the actual measured ones.

关键词： reinforced concrete     steel truss     core walls     seismic performance    

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Effects of inclination angles of disc cutter on machining quality of Nomex honeycomb core in ultrasonic

Yidan WANG, Renke KANG, Yan QIN, Qian MENG, Zhigang DONG

《机械工程前沿（英文）》 2021年 第16卷 第2期   页码 285-297 doi: 10.1007/s11465-021-0631-9

摘要： Ultrasonic cutting with a disc cutter is an advanced machining method for the high-quality processing of Nomex honeycomb core. The machining quality is influenced by ultrasonic cutting parameters, as well as tool orientations, which are determined by the multi-axis machining requirements and the angle control of the cutting system. However, in existing research, the effect of the disc cutter orientation on the machining quality has not been studied in depth, and practical guidance for the use of disc cutters is lacking. In this work, the inclined ultrasonic cutting process with a disc cutter was analyzed, and cutting experiments with different inclination angles were conducted. The theoretical residual height models of the honeycomb core, as a result of the lead and tilt angles, were established and verified with the results obtained by a linear laser displacement sensor. Research shows that the residual height of the honeycomb core, as a result of the tilt angle, is much larger than that as a result of the lead angle. Furthermore, the tearing of the cell wall on the machined surface was observed, and the effects of the ultrasonic vibration, lead angle, and tilt angle on the tear rate and tear length of the cell wall were studied. Experimental results revealed that ultrasonic vibration can effectively decrease the tearing of the cell wall and improve the machining quality. Changes in the tilt angle have less effect than changes in the lead angle on the tearing of the cell wall. The determination of inclination angles should consider the actual processing requirements for the residual height and the machining quality of the cell wall. This study investigates the influence of the inclination angles of a disc cutter on the machining quality of Nomex honeycomb core in ultrasonic cutting and provides guidelines for machining.

关键词： Nomex honeycomb core     disc cutter     inclined ultrasonic cutting     machining quality    

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Construction of sustainable, colored and multifunctional protein silk fabric using biomass riboflavin

《化学科学与工程前沿（英文）》 2023年 第17卷 第8期   页码 1131-1139 doi: 10.1007/s11705-023-2321-0

摘要： Riboflavin sodium phosphate has been confirmed as a promising biomass product derived from natural plants. In this paper, a novel method of dyeing and multifunctional modification of silk fabric by impregnation with riboflavin sodium phosphate was proposed, such that protein silk fabric can be endowed with bright yellow color and multi-functionality. The results of this paper confirmed that the pH and concentration of riboflavin sodium phosphate solution are critical factors for dyeing and multifunctional modification. Attractively, the photochromic performance was one of the most distinctive features of the modified silk fabric, and the dyed silk fabric turned into fluorescent green from original yellow under 365 nm ultraviolet lamp. Furthermore, the modified silk fabric exhibited good antibacterial properties with a high inhibition rate of 92% for Escherichia coli. Besides, the flame retardancy of silk fabric was significantly improved after modification. The damaged length of modified silk fabric with 40% owf riboflavin sodium phosphate was lower than 10.4 cm and passed the B1 classification. As revealed by the result of this paper, riboflavin sodium phosphate is sufficiently effective in serving as an eco-friendly multifunctional agent for strengthening the add-value of silk textiles.

关键词： biomass     riboflavin sodium phosphate     silk protein     multifunctional modification     flame retardant    

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Antithrombin deficiency and decreased protein C activity in a young man with venous thromboembolism:

null

《医学前沿（英文）》 2018年 第12卷 第3期   页码 319-323 doi: 10.1007/s11684-017-0553-4

摘要：

Antithrombin and protein C are two crucial members in the anticoagulant system and play important roles in hemostasis. Mutations in and lead to deficiency or dysfunction of the two proteins, which could result in venous thromboembolism (VTE). Here, we report a Chinese 22-year-old young man who developed recurrent and serious VTE in cerebral veins, visceral veins, and deep veins of the lower extremity. Laboratory tests and direct sequencing of and were conducted for the patient and his family members. Coagulation tests revealed that the patient presented type I antithrombin deficiency combined with decreased protein C activity resulting from a small insertion mutation c.848_849insGATGT in and a short deletion variant c.572_574delAGA in . This combination of the two mutations was absent in 400 healthy subjects each from southern and northern China. Then, we summarized all the mutations of the and gene reported in the Chinese Han population. This study demonstrates that the combination of antithrombin deficiency and decreased protein C activity can result in severe VTE and that the coexistence of different genetic factors may increase the risk of VTE.

关键词： antithrombin deficiency     protein C activity     mutation     variant     venous thromboembolism     anticoagulants    

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Improving performance portability for GPU-specific OpenCL kernels onmulti-core/many-coreCPUs by analysis-based

Mei WEN,Da-fei HUANG,Chang-qing XUN,Dong CHEN

《信息与电子工程前沿（英文）》 2015年 第16卷 第11期   页码 899-916 doi: 10.1631/FITEE.1500032

摘要： OpenCL is an open heterogeneous programming framework. Although OpenCL programs are functionally portable, they do not provide performance portability, so code transformation often plays an irreplaceable role. When adapting GPU-specific OpenCL kernels to run on multi-core/many-core CPUs, coarsening the thread granularity is necessary and thus has been extensively used. However, locality concerns exposed in GPU-specific OpenCL code are usually inherited without analysis, which may give side-effects on the CPU performance. Typically, the use of OpenCL’s local memory on multi-core/many-core CPUs may lead to an opposite performance effect, because local-memory arrays no longer match well with the hardware and the associated synchronizations are costly. To solve this dilemma, we actively analyze the memory access patterns using array-access descriptors derived from GPU-specific kernels, which can thus be adapted for CPUs by (1) removing all the unwanted local-memory arrays together with the obsolete barrier statements and (2) optimizing the coalesced kernel code with vectorization and locality re-exploitation. Moreover, we have developed an automated tool chain that makes this transformation of GPU-specific OpenCL kernels into a CPU-friendly form, which is accompanied with a scheduler that forms a new OpenCL runtime. Experiments show that the automated transformation can improve OpenCL kernel performance on a multi-core CPU by an average factor of 3.24. Satisfactory performance improvements are also achieved on Intel’s many-integrated-core coprocessor. The resultant performance on both architectures is better than or comparable with the corresponding OpenMP performance.

关键词： OpenCL     Performance portability     Multi-core/many-core CPU     Analysis-based transformation    

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标题 作者 时间 类型 操作

Construction and expression of hepatitis B virus vector encoding TC-tagged core protein

Yuanyuan LIN MD, Xiaoming CHENG MS, Yuhu SONG MD, Peiyuan LI MD, Ying CHANG MM, Jinjian YAO MD, Jusheng LIN MD, PhD, Li ZHOU PhD, Leiming XU PhD,

期刊论文

脂肪变性诱发肝癌发生机制——HCV核心基因转基因小鼠的经验教训

Pan Diao, Fangping Jia, Xiaojing Wang, Xiao Hu, Takefumi Kimura, Takero Nakajima, Toshifumi Aoyama, Kyoji Moriya, Kazuhiko Koike, Naoki Tanaka

期刊论文

知识发现领域中当今面临的五类重大问题

杨炳儒

期刊论文

Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

期刊论文

Postprocessor development for ultrasonic cutting of honeycomb core curved surface with a straight blade

期刊论文

Establishment and verification of a shrinking core model for dilute acid hydrolysis of lignocellulose

Cunwen WANG, Xiaoling DUAN, Weiguo WANG, Zihao LI, Yuanhang QIN

期刊论文

Recent advances in gold-metal oxide core-shell nanoparticles: Synthesis, characterization, and their

Michelle Lukosi,Huiyuan Zhu,Sheng Dai

期刊论文

The role of protein kinase C epsilon in neural signal transduction and neurogenic diseases

null

期刊论文

Property-performance relationship of core-shell structured black TiO photocatalyst for environmental

期刊论文

Wheat gluten protein and its impacts on wheat processing quality

Wujun MA, Zitong YU, Maoyun SHE, Yun ZHAO, Shahidul ISLAM

期刊论文

Experiment and calculation on seismic behavior of RC composite core walls with concealed steel truss

Wanlin CAO , Weihua CHANG , Changjun ZHAO , Jianwei ZHANG ,

期刊论文

Effects of inclination angles of disc cutter on machining quality of Nomex honeycomb core in ultrasonic

Yidan WANG, Renke KANG, Yan QIN, Qian MENG, Zhigang DONG

期刊论文

Construction of sustainable, colored and multifunctional protein silk fabric using biomass riboflavin

期刊论文

Antithrombin deficiency and decreased protein C activity in a young man with venous thromboembolism:

null

期刊论文

Improving performance portability for GPU-specific OpenCL kernels onmulti-core/many-coreCPUs by analysis-based

Mei WEN,Da-fei HUANG,Chang-qing XUN,Dong CHEN

期刊论文